ვირთაგვების ქცევით და მორფოლოგიურ მახასიათებლებზე რკინის ნანონაწილაკებთან დაკავშირებული ქვერცეტინის ეფექტების შესწავლა

The study of effective remedies against neurodegenerative disorders showed neuroprotective nature of flavonoids - an antioxidant of plant origin. Quercetin is one of the widespread representatives of this group, but the use of Quercetin has been limited due to it poor bioavailability. The modern approach to solve problem is usage of nanoparticles (NP). It is shown that nanoparticles protect, control the release and increase the influence of bioactive compounds to the target area. Nanoparticles can cross blood-brain barrier (BBB). In case of using magnetic iron oxide nanoparticles (MNP) as a drug delivery system, the orientation and location of particles could be controlled by external magnetic field, which is characterized by good bioavailability. Thus, it was suggested that exposure of the magnetic field to the brain could have impact on the transport of drugloaded MNP.
The aim of our study was to investigate the effects of intravenous injected Quercetin-loaded magnetic iron oxide nanoparticles (Q-MNP) on behavioral characteristics unilateral exposure of the magnetic field (1 tesla, 1 hour) on the brain (temporal lobe projection) and correlatives of morphological identification.
Experiments were conducted on wild type white laboratory rats (100-120gr). Rats behavioral experiments were performed in the open field and T-maze tests. Q-MNPs were injected in the tail vena under unilateral external magnetic field (1 tesla) exposure to the temporal lobe projection of the rat brain.
In morphological experimental the administration of the Q-MNP were made after 30 minutes of the magnetic field exposure, and the extirpation of the brain were performed after 80-120 min of the magnetic field removal. Perl’s Prussian blue stain was used to determine the iron content in brain structures.