ღეროვანი უჯრედებისა და ოსტეოპლასტიური მასალების კომბინირებული გამოყენება, ყბა-სახის ტრავმული დაზიანებების დროს

Reconstruction of mandibular defects is a challenging issue in maxillofacial surgery.
Success of the treatment is defined by the process of reparative regeneration of bone tissue,
which is often acompanied with wound infection, failure of microcirculation and tissue
hypoxia. Aim of our project was to demonstrate the advantage of osteoplastic material and
bone marrow derived mesenchymal stem cells combination in regeneration of lower jaw
bone defect. For this reason, mandibular bone defect was created in laboratory rats.
Experimental treatment of animals was performed in different ways: I series: group 1. –
Bone defect was regenerated without any treatment, group 2. – Bone defect was filled with
osteoplastic material (BIO-OSS), group 3. – Bone defect was filled with BIO-OSS, which was
previously infiltrated by bone marrow mesenchymal stem cells. II series: group 1. - C57BL/6-
GFP Line Mice Donors used for obtaining autologous bone marrow stem cells; group 2. -
C57BL / 6 line mice for modeling of lower jaw defects. Reconstruction with bone mineral
(BIO-OSS) and of autologous bone marrow stem cells derived from the C57BL / 6-GFP line
mice.
Animals were under observation for 6 months. Durring this period bone regeneration
was comparatively analysed in these tree experimental groups. Observational, histochemical
and x-ray research methods were used in this study. Our results suggest that use of
osteoplastic material and mesenchymal stem cells combination can increase formation of red
bone marrow, which is normally slow under natural condition. Therefore, we can conclude
that application of osteoplastic material with bone marrow derived mesenchymal stem cells
can decrease time of bone defect osteointegration and define the quality of regenerated bone
tissue.