ადამიანის ქორიონული გონადოტროპინის სპეციფიურობის ბუნებრივი ანტისხეულების შესწავლა საკვერცხის კეთილთვისებიანი და ავთვისებიანი სიმსივნეების მქონე ავადმყოფებში.

Background: Ovarian tumours occur during the reproductive age or after menopause and can be subdivided into benign, borderline or malignant. Twice as many patients with malignant ovarian tumous were found to be seropositive for human chorionic gonadotropin (hCG) as compared to benign tumors. It has been demonstrated high titres of natural anti-hCG IgG antibodies in the sera of patients with benign ovarian tumours.
In this study we further characterize the titres and subclass usage of naturally-occurring IgM and IgG antibodies against hCG and its subunits in patients with ovarian cyst and ovarian adenocarcinoma
Materials and Methods: Titration of blood sera from 37 female patients (aged 22-61 after ethical permission and informed consent) diagnosed with ovarian cyst (n=17), ovarian adenocarcinoma (n=10) and healthy age-matched controls (n=10) was performed using indirect enzyme-linked immunosorbent assay (ELISA). Binding of the sera to the following antigens was tested: hCGαβ, hCGβ, hCGα, hCGβ C-terminal peptide (hCGβCTP) and hCGβ core fragment (hCGβCF). For identification of IgG-subclasses the plates were coated with the antigens mentioned above. Following the application of an optimal serum dilution, defined as the 50% titre, rabbit –horseradish peroxidase (HRP) conjugated antibody (Sigma, USA) to human IgG subclasses (IgG1, IgG2, IgG3 and IgG4) were added at a concentration 1µg/ml. The plates were read at the optical density (OD) 450 nm in a spectrophotometer (Selecta, Spain).