ევროპული წაბლის (Castanea sativa) ზიგოტური ემბრიონის კრიოპრეზერვაცია დეჰიდრატაციის და ვიტრიფიკაციის გამოყენებით

The conservation of plant biodiversity is an important issue concerning the human population world wide. Conservation plays a fundamental role in maintaining plant species of ecological and economic importance. Chestnut (Castanea sativa Mill.) is the dominant of mountainous forests of Western Georgia. Chestnut in the Red List of Georgia IUCN category is represented by vulnerable status (VU). Traditional ways of chestnut reproductions are complex and full of drawbacks and one of the most important ways is its long-term conservation at ultra low temperature (-196 °C) in liquid nitrogen. Freeze-preservation is the only technique, which guarantees the possibility of the problematic species, such as non-orthodox seed, also vegetatively plants, of safe and lowest long-term conservation. Under these conditions, biochemical and most physical processes are completely arrested. As such, plant material can be stored for unlimited periods.